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當前位置:首頁公司新聞制備色譜的全新方法詳介

制備色譜的全新方法詳介

更新時間:2012-05-10點擊次數(shù):1763
制備色譜的全新方法詳介
  高速逆流色譜★(high-speedcountercurrentchromatography,HSCCC)是20世紀80年代發(fā)展起來的一種連續(xù)的液—液分配色譜分離技術,它不用任何固態(tài)的支撐物或載體。它利用兩相溶劑體系在高速旋轉(zhuǎn)的螺旋管內(nèi)建立起一種特殊的單向性流體動力學平衡,當其中一相作為固定相,另一相作為流動相,在連續(xù)洗脫的過程中能保留大量固定相。由于不需要固體支撐體,物質(zhì)的分離依據(jù)其在兩相中分配系數(shù)的不同而實現(xiàn),因而避免了因不可逆吸附而引起的樣品損失、失活、變性等,不僅使樣品能夠全部回收,回收的樣品更能反映其本來的特性,特別適合于天然生物活性成分的分離。而且由于被分離物質(zhì)與液態(tài)固定相之間能夠充分接觸,使得樣品的制備量大大提高,是一種理想的制備分離手段。它相對于傳統(tǒng)的固—液柱色譜技術,具有適用范圍廣、操作靈活、、快速、制備量大、費用低等優(yōu)點。目前HSCCC技術正在發(fā)展成為一種備受關注的新型分離純化技術,已經(jīng)廣泛應用于生物醫(yī)藥、天然產(chǎn)物、食品和化妝品等領域,特別在天然產(chǎn)物行業(yè)中已被認為是一種有效的新型分離技術;適合于中小分子類物質(zhì)的分離純化。我國是繼美國、日本之后zui早開展逆流色譜應用的國家,俄羅斯、法國、英國、瑞士等國也都開展了此項研究。美國FDA及世界衛(wèi)生組織(WHO)都引用此項技術作為抗生素成分的分離檢定,90年代以來,高速逆流色譜被廣泛地應用于天然藥物成分的分離制備和分析檢定中。

經(jīng)營:濃縮儀,溶劑蒸發(fā),凍干機

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